The player controls Roger Rabbit through four levels, each with its own specific task to complete.
The first level is a race-car type game between Roger Rabbit, driving Benny The Cab and the Toon Patrol. The second level is in the Ink & Paint Club as Roger must run around the nightclub's tables collecting the various pieces of paper that the penguin waiters set down, without getting kicked out because of the pieces of paper are Marvin Acme's will. The third level is another racing scenario and the final level involves you doing various gags in the Acme factory to kill the Weasels and save Roger's wife, Jessica.
The box also includes a short catalog of items available at the Acme factory . This gives clues as to what to use in playing the final level.