The Vampires first appeared during the song "This is Halloween". After the celebration, the Mayor announced they had won the award for the blood drained in a single evening. Upon the temporary departure of Jack Skellington, they attempt to locate Jack within Halloween Town, with one of the brothers commenting at having looked for him behind the Cyclop's eye. They are later among the various other Halloween Town residents during Jack's town meeting regarding information of Christmas. Later during the song "Making Christmas" as the Halloween Town residents are making gifts, they were shown creating the vampire Duck Toy along with the Vampire Teddy bear, with the intention of these being toys for the children of Christmas Town to enjoy, unaware of their frightening nature to the children. Along with the other residents, they watch Jack's venturing as Santa Claus over Christmas Town through scrying the town well's waters, and are distraught upon his seeming demise. Upon Jack's return to Halloween Town, they are delighted to see him again, and are later shown playing croquet in the snow together, following Santa Claus snowy spell over Halloween Town.
Little is known regarding their individual personalities, but like all the other Halloween Town residents, they are benevolent and harmless individuals with a deep fondness and appreciation for their King, Jack Skellington. They are shown to be calm, stoic and inquisitive individuals, who are interested in seeking knowledge and for discovering new ways of celebrating their holiday Halloween. They are all also shown to have a certain creative and artistic streak to them, given their creation of both the Duck Toy and Vampire Teddy, with the intention of these beings toys to be loved by the children of Christmas Town , despite their creations' monstrous and malevolent nature.
Video Game AppearancesThe Nightmare Before Christmas: The Pumpkin King.
The Vampire Brothers appear in the video game The Nightmare before Christmas: Oogie's revenge, after being transformed into their original bat forms by Oogie Boogie, who also possess the necessary pieces to completing the Mayor's courtyard.