Twiga is one of the animals who sings the special song for the Kupatana Celebration. She is shown rehearsing the song with several other inhabitants of the Pride Lands, including Basi and Mbuni. When the time of Kupatana arrives, Twiga sings the song as the animals assemble. After she and the other animals finish, Simba makes a small speech, and the baobab tree's flowers bloom. The celebration, however, is interrupted by Reirei. Twiga and the other giraffes are attacked by the jackal pups, but Beshte drives them away.
Upon spotting Twiga feeding from the trees, Ajabu dashes into hiding. Twiga greets Beshte cordially, and Beshte greets her in return. When Ajabu returns, Beshte remarks that there is no need to hide from Twiga, as she is friendly. Twiga briefly appears during the song Life in the Pride Lands. She nuzzles a giraffe calf before walking away, followed by the calf and another two giraffes.
Twiga is walking along when Ono flies abruptly in front of her. The egret apologizes and advises her against continuing on the trail that she is on. She tells him that she must get to Big Springs, but Bunga informs her that a mudslide has blocked the path. Twiga realizes what he means and thanks him for the heads-up, a statement Bunga finds hilarious.
Twiga and the other giraffes' water hole has been blocked by boulders. The Lion Guard arrives to sort things out, and, noticing that Ono has lost his crest feathers, Shingo calls him an "egghead." Ono initially believes this to be a reference to his intelligence, but Twiga bends down and tells him that it's because his head looks like an egg, laughing with Shingo. Bunga defends his friend, insisting that his head looks more like a hippo's bottom, which only results in more laughter from the two giraffes. When the boulders are removed, she goes to the water hole and drinks with her herd.
Twiga tries to get a Drink from the Watering Hole, but needs to keep her Daughter, Juhudi, Save and, asks Muhimu where Hamu is. She drops her Daughter off at Hakuna Matata Falls. She later picks up Juhudi when the Jackals are defeated.
Twiga picks up her daughter, Juhudi, from Bunga at Hakuna Matata Falls
Twiga and Vuruga Vuruga mutter about Makuu
Twiga and Vuruga Vuruga act suspiciously about Makuu in front of the Lion Guard
Twiga wonders where Hamu is
Twiga and Zazu
Twiga notices Simba
Twiga during My Own Way
Twiga watches Bunga laugh
Twiga and Ono
Twiga laughs at Ono
Twiga greeting Beshte
Twiga and a young giraffe
Twiga nuzzling the giraffe calf
Twiga singing with the other animals.
Under attack by the jackals
Twiga wishes to talk
A casual Twiga
Twiga and Vuruga Vuruga reveal all
Believing that Makuu is dead because of their trap, Twiga and Vuruga Vuruga own up and apologize