Professor Owl teaches his class about the elements of musical instruments, melody and inspirations for songs. The show is primarily made up of footage from Melody and Toot, Whistle, Plunk and Boom, as well as new animation bookending other Disney cartoon musicals.
- Bill Thompson - Professor Owl, Bertie Birdbrain
- Scenes from this episode were re-used in Disney Sing Along Songs: Heigh-Ho, Disney Sing Along Songs: Zip-a-Dee-Doo-Dah, and Disney Sing Along Songs: Fun with Music.
- The unnamed boy in A Cowboy Needs a Horse was given a name: Johnny.
- The new character Roberta Robin was introduced and was the sister of Penelope Pinfeather.