The Toon Shoe appears among other ones during the investigation scene at the Acme Factory, when a crate full of them is accidentally knocked open. It apparently likes the detectives and escapes from being returned to the crate. However, when it later approaches Judge Doom, he uses it to show the detectives how his Dip works to kill off toons. It is last shown melting into paint while screaming and whimpering in agony upon making contact with the Dip. It isn't seen again for the rest of the film.