In the film, the Toon Patrol are the law enforcers of Toontown, although they behave less like law enforcers and more like gangsters and crooks due to their behaviors. Judge Doom has hired them to arrest Roger Rabbit for the murder of Marvin Acme. Their vehicle is a real-life black 1937 Dodge Humpback panel truck which serves as a paddy wagon. The vehicle is fitted with official City of Los Angeles "Toon Patrol" decals on the front doors.
Like all the other Toons in the movie, the Toon Patrol are invincible to physical body harm except the chemical blend called "The Dip."
In the case of the weasels, it is shown that prolonged laughter is also lethal to them; while Eddie Valiant jokes around in front of them during the climax of the film, all except their leader Smarty die from laughing at him; Smarty meets his demise after Valiant crotch-kicks him into a vat of The Dip. Whether or not the weasels knew that Doom was a Toon himself is never confirmed, but their relationship with him may hint that they had no knowledge of such.
- Smarty is the leader of the Toon Patrol, and is ranked a sergeant by Judge Doom. He has brown fur with tan markings and wears a light pink double-breasted zoot suit coat with a gold chain in the left pocket, a white dress shirt with a hot pink bed-jewelled tie, a matching hat, and spats on his feet. His weapon of choice is a revolver, though in the bar scene he is shown threatening Eddie with a switchblade. He is the smartest, cleverest, and wittiest of the weasels. He is also arrogant, bossy, and a bit fussy. Although very sarcastic, he is the straight-man of the group, possessing the most self-control and least "toony" attitude. While he is very antagonistic in the film, he seems to care about the other members of the Toon Patrol, always being the one to warn them about what might happen to them if they can't control their laughter. However, like them, he still enjoys a good laugh, himself. He commits a malapropism almost every time he speaks, such as when he says that a tip on Roger's whereabouts has been "corrugated" ("corroborated") by several sources. He is the third weasel to die at the end of the film, although not by losing control of his laughter. Upon disagreeing with a lyric in the song Eddie "entertains" the weasels with, Eddie kicks him in the crotch, sending him flying into the mixer on the Dip machine, where he dissolves and dies. Unlike the other weasels, his ghost doesn't appear; this is because the dip is meant to completely destroy a toon so there would be no toon ghost.
- Greasy is allegedly the runner-up for leader of the Weasels and Smarty's second-in-command. He is Puerto Rican and has long greasy black hair and dark brown fur. He wears a green zoot suit styled trench coat and trousers hiked up to his chest, partially obscuring a pink tie and white dress shirt, and spectator shoes; he appropriately resembles a zoot suit gangster from the L.A. "Zoot Suit Riots" of the 1940s. Like his compatriot Stupid, Greasy is overweight, although this does not appear to have affected his self-esteem. Of all the weasels in the film, Greasy is most eager to fight, and yields a switchblade as his weapon of choice, though he is also seen carrying a semi-automatic pistol while investigating Eddie's office. Greasy is mostly shown speaking English with a strong Puerto Rican accent, but he curses in Spanish when a bear trap hidden in Jessica's cleavage clamps down on his hand as he frisks in between her breasts, and when Roger shoots into the Acme Factory via a storm drain, carrying Greasy (who was standing on top of it) with him towards the ceiling. He appears perverted, as he quickly takes Judge Doom's orders to search Jessica for Marvin Acme's will as an opportunity to feel her and immediately goes for her cleavage. Greasy is the fourth weasel to die after losing control of his laughter and drops dead out of the cab of the Dip machine. He also made the Dip machine move because his foot came off the brake when he fell out of the driver's seat as he died from laughter.
- Psycho is the most insane weasel in the gang. He wears an unbuckled straitjacket, has blue eyes with yellow and white swirls and his weapon of choice is a straight razor. He only has two lines in the film; he first speaks as he prepares to shoot dip at Roger and Jessica, when he says, "Time to kill the rabbit. heheheheh." and when he says, "Bye-bye." after he dies. He gets excited by the concept of killing, as he is heard gleefully laughing when Judge Doom kills the toon shoe in the bucket of dip; he is nearly drooling in anticipation as Smart Ass attempts to drive into Benny the cab, and he takes it upon himself to shoot the dip at Roger and Jessica. On a few occasions, it is implied that he enjoys pretending his straitjacket is buckled by hugging himself tightly, namely when he exits the Toon Patrol's car in his first appearance, and while laughing after Eddie stuffs a bar of soap into Smart Ass's mouth, though this might also be implying that he just might not be entirely used to having the straitjacket unbuckled. He remained a threat after his death; he lost control of his laughter and hit the switch that turned on the Dip nozzle before he lost his balance and fell off the platform. In an attempt to save himself, he hung on to the yellow handle, which pulled it down, making the nozzle turn left, but he still slipped off into the spinning brushed below. His soul rose up like the others, but instead of just doing nothing he turned the pressurized dip spray back at Roger and Jessica. He is the last weasel to die, not by losing control of his laughter, but by losing his footing and dropping on the giant rotating brush of the Dip Machine were he might have got flatten to death and later dissolved in dip.
- Wheezy is the smoking weasel, and has a mouth full of several smoking cigarettes. He wears a dingy white mashed bowler hat in which he carries an additional stash of cigarettes, a black vest, a loose tie, and a wrinkled shirt. His weapon of choice is a Tommy Gun but he only uses it once in the film (when he shoots around Eddie Valiant's doorknob to open the door), preferring to use physical combat. He appears afraid of dying, as he is seen unsuccessfully trying to pull his soul back into his body, although when it breaks free, it does not appear regretful. He has bluish-grey fur while his comrades have brown fur. If he doesn't have cigarettes in his mouth, he speaks in a deep voice, and if they are in his mouth, he has a very raspy voice. He speaks twice in the film; after Roger and Eddie free Benny the Cab, and in the Acme Factory while tying Roger and Jessica up. He is the second weasel to die after losing control of his laughter and also presumably from too much smoking. He is the most mellow of the weasels, only seeming to really get worked up when his soul tries to leave his body.
- Stupid is the dumbest member of the gang. He is clearly overweight, while all the other weasels are fairly slim. He wears a blue and gray striped T-shirt, a red beanie with a propeller and dingy blue and white sneakers with untied laces. His weapon of choice is a baseball bat with a nail through it. He is so stupid that he is seen hitting himself over the head with his own weapon while laughing. He has one line in the film when he excitedly informs Judge Doom that Toontown is on the other side of the wall in the Acme Factory, which he and Wheezy are drilling open. He is the first weasel to die after losing control of his laughter and his body is seen clutching a lily.
- During production, there were going to be seven weasels rather than five, much like the Seven Dwarves. The other two were named "Slimy" and "Flasher." Other names considered were Crazy, Sleazy, Scummy, Itchy, and Twitchy. However, only Slimy and Flasher made it to the sketch stage and no voice work for them was ever completed. Slimy was a black weasel with 1950s greaser attire who puked slime (and, just by looking at his concept art, could also be playing in it) and carried a blackjack as a weapon. Flasher, as his name implies, wore a trenchcoat, but did not carry a visual weapon. The conceptual art of these characters is included in the 15th anniversary DVD edition of Who Framed Roger Rabbit?.
- Also, there is an unnamed weasel in Roger Rabbit's Car Toon Spin. He is a dark brown weasel who wears a gray baggy hat, a white button-up shirt, and dark cadet-blue baggy suspenders. He was voiced by Will Ryan. His origins are unknown, though some names have been suggested by fans, notably "Sleazy."