She is a lioness cub in the Pridelander pride led by King Simba and Queen Nala. She along with Zuri is a friend of Simba and Nala's daughter Kiara.
Tiifu is kind, caring, and patient, follows rules and trusts the judgment of her friends. She is rather curious, going so far as to ask questions even after being warned against doing so. She admires Kiara and holds her title as future queen in high regard. She also seems to be quite defensive of her friendship with Kiara as she became jealous when Fuli went to Kiara's side when tracking gazelles.
Although she is intelligent, like her best friend Kiara, Tiifu is arrogant, immature, cheerful, carefree, laid-back and reckless.
Tiifu first appears when she and Kiara see Zuri trying to pry her claws out of a log (after attempting to sharpen them) and sees her being freed by Timon and Pumbaa. She later listens to Bunga when he reveals that Kion is to lead the new Lion Guard with Bunga being the first member picked. Tiifu later watches Bunga sing "Zuka Zama".
Tiifu is later seen with Kiara when they join Simba and Zazu when Kiara shows Simba proof that Kion put Bunga in The Lion Guard. Simba thanks, Kiara before sending her and Tiifu to track gazelles while he has a word with Kion.
Tiifu and Kiara are later seen tracking a herd of gazelles with Kiara telling Tiifu that they mustn't take more than their fair share as it would break The Circle of Life. After noticing Janja and his clan invading the Pride Lands, Kiara sends Tiifu to fetch Simba. Tiifu later returns with Simba, Nala and Rafiki where they watch in horror as Kiara gets caught up in a stampede Janja and his clan have started amongst the gazelles. Luckily, Kion, Bunga and the other Lion Guard members Beshte, Fuli and Ono come to Kiara's rescue and get her to safety, to the relief of Tiifu, Simba, and Nala.
Tiifu and Zuri serve as advisors to Kiara after she is made temporary Queen of the Pride Lands when Simba and Nala leave to attend Amanifu's funeral. However, when Kiara leaves to have a supposed peace treaty with Janja, Tiifu and Zuri stay behind, believing that their friend would be fine on her own, causing them to be criticized by Kion, who leaves with the Lion Guard to save his sister.
Tiifu joins Zuri, Kiara, and Nala in tracking gazelles in preparation for Kiara's first hunt. Tiifu becomes jealous when Fuli sides up next to Kiara, believing that Fuli is trying to be Kiara's new "bestie".
Tiifu and Zuri try to make Kiara look her best for the Royal Buffalo Wallow, which she is to open on Simba and Nala's behalf. However, the trio end up stranded on a island when a flood strikes the Pride Lands. With the help of the Lion Guard, the trio get off the island and go to the wallow.
Tiifu comforting Zuri
Tiifu, Zuri, Kiara and Kion
Tiifu, Zuri, Kiara and Bunga.
Tiifu, Timon and Zuri
Tiifu with Kiara, Zuri, Kion, Bunga, Timon and Pumbaa
Tiifu, Nala and Simba watching in horror as Kiara is caught in a gazelle stampede
Tiifu, Kiara, Nala, Simba and Rafiki
Tiifu watching Makuu and Pua's mashindano
Tiifu, Kiara and Zuri being taught to track gazelles by Nala