In the movie, The Thugs are Ratigan's henchmen. All are mice (except for one, who is a lizard). They are very loyal to Ratigan, and will do anything to help him, including overthrowing the Queen and becoming ruler and guards of Mousedom. Ratigan's Thugs love to drink and sing about their boss, and they also boo Basil of Baker Street, who has interfered with their boss' plans. During the song, one of the thugs (named Bartholomew) accidently calls Ratigan a rat, and Ratigan has him fed to his cat, Felicia, while three of the thugs watched sadly. They soon recover and sing about their boss again, crowing him with a crown, awaiting to help him succeed with his evil plan. Later on, when Basil and Dawson sneak into the lair, Ratigan's bat henchman, Fidget, dresses up as Olivia (the girl they're looking for) and, when Dawson taps on the bottle Olivia is in, Fidget turns around and smacks his lips, and the Thugs and Ratigan appear and laugh at Basil and Dawson in their pirate disguises. The thugs then tie Basil and Dawson in a trap and leave for Buckingham Palace dressed in guard uniforms. At the palace, The Thugs manage to ambush the real Queen's Guards and enter the Queen's room, delivering her a present (which is a Robot Version of herself) and then Ratigan appears and orders his Thugs to take The Queen away, which they do. The Thugs act like the real Queen's Guards during her speech, during which, they manage to fool the crowd with the Robot Queen, which announces Ratigan as her new Royal Counciler. The lizard thug manages to give notes for Olivia's father, Mr. Flaversham, to read into the robot Queen's speaker voice to say to the crowd. Luckily, Basil and Dawson and Olivia manage to escape the trap and bottle and manage to arrive at the palace and save the Queen and fight off Ratigan's thugs and tie them up, where they are arrested. If they are hanged or not remains unknown.
Ratigan's Thugs sometimes appeared alongside Ratigan as guests in the TV show House of Mouse.