In Mickey Mouse, Spike returns in "Bee Inspired" where he repeatedly frightens Mickey, keeping Minnie from finishing her painting. In the end, they become friends and Spike saves Mickey from an angry swarm of his companions by giving a dramatic speech (all in buzz language with subtitles).
Spike the Bee made a handful of appearances in Disney comics. In the 1948 Inferior Decoratorhe attacks Donald's house with a whole swarm of bees because Donald's new wallpaper represents flowers.
The Bee enemy design in DuckTales Remastered resembles Spike's original design from "Window Cleaners."