Slue-Foot Sue is a redhead in braids from another part of her world. She is a thrill-seeking daredevil with no fear or shame for anything. In the end, she was self-shot to the moon where she stays due to Bill's horse Widowmaker, as well as her decision to wear a bustle while riding said horse.
Slue-Foot Sue is presented as a catfish riding cowgirl. She captures the attention of Pecos Bill and the two fall in love. The two eventually make plans to marry. The day of the ceremony, Sue dresses in a beautiful yellow wedding dress with a huge steel-spring bustle, and insists on riding Widowmaker, Pecos Bill's horse. However, Widowmaker is jealous of Sue, because she had taken away Bill's attention from him. As soon as Sue got on Widowmaker's back, he attempts to throw Sue off in a fit of rage. Admirably, she is able to stay on the bucking horse for quite a while. But because of the bustle she wears, Sue starts bouncing out of control and ends up shooting herself high into the air. Her bustle continues to bounce her higher each time she lands on it, and Bill attempts to rescue her with his lasso, but barely misses (It's implied that Widowmaker interfered with the attempt). At night, Sue finally lands on the moon, unable to return to earth, leaving Bill to mourn her loss. The narrator then adds that this is why coyotes howl at the moon.
Slue-Foot Sue appears in the episode "House of Crime".
A live version of Slue-Foot Sue, originally played by Betty Taylor, was the host of the Golden Horseshoe Revue and the owner of the Golden Horseshoe Saloon. She performed songs such as the Golden Horseshoe Revue theme song, Bill Bailey and the Pecos Bill song from the film, during which Pecos himself enters the show.
The animated version of the character also appears in window decorations to the saloon to the present day as well as on the stage curtains in the days of the original show.