The Skeletons are the main protagonists of the short. The first one rises from behind a grave and scares away two cats, before being joined by three more. They proceed to dance and frolic in the graveyard, in general good-hearted dances and musical games, though they are somewhat reckless and unintentionally harmful in their behavior (they hurt a cat when attempting to use his whiskers like a violin, and one of them wrecks another using him as a piano). When the rooster cries, the Skeletons panic (probably because, as in common ghost lore, they cannot stand sunlight) and merge into a single several-limbed creature that hides in a large tomb.
When Mickey Mouse enters a haunted house, he is repeatedly scared by the Skeletons (who are more numerous than in their debut). He eventually comes across their leader, a hooded skeleton with a missing tooth (sometimes thought to be the Grim Reaper, though he isn't identified as such). Surprisingly, as he is cornered, Mickey is simply told to play the piano for them, and the Skeletons begin dancing and frolicking in the haunted halls, much like in The Skeleton Dance. Mickey, still scared, tries to escape the house, pursued by several Skeletons (and coming across more of them).
The Mad Doctor's large castle is haunted by several Skeletons. Most of them are hiding in the furniture, including in the lift, cupboard, inside a stone pillar and inside the steps of a staircase (which are apparently really coffins). They attack Mickey, though whether they inhabit the Caslte and have decided to get rid of trespasser, or are doing so on the Mad Doctor's orders, is left unclear. A skeleton spider (an obvious nonsensicality, seeing how spiders are invertebrate, which could however be explained by the Spider being a creation of the Doctor's) also appears and attempts to eat Mickey.
The skeletons make cameo appearances in several episodes, including "Dennis the Duck", "House Ghosts" and "Halloween With Hades". In Dennis the Duck, they are among the black-and-white cartoon stars invited to the House for a special celebration along with Dennis himself. In House Ghosts, they help the ghosts of the Haunted Mansion and the Lonesome Ghosts scare Pete during a Grim Grinning Ghosts song number.
Video game appearances
The skeletons are frequent enemies inthe "Mad Doctor" level, mirroring their inhabiting the Doctor's castle in the cartoon.
Some Skeletons are seen in the projector reel transition level based on the "The Mad Doctor" cartoon. Hidden-unseen skeletons eeriely lift up the stairs that can make Mickey fall down and another towards the end will throw a piece of brick at you and will cause you to lose some health. Some others appear in the second and third transition levels themed to "The Haunted House".
In Lonesome Manor, Mickey confronts minions of the Mad Doctor who resemble Bashers but, instead of being those of pirates, their toon parts look like the skeletons. Since the skeletons were already the Mad Doctor's minions in his debut cartoon, it's possible he turned them into bashers just like he did the pirates of Tortooga.
The skeletons appear in the two transition levels themed to the 1929 short that they debuted in. The original cartoon also appears on the game disc as an extra.
- The hooded skeleton in The Haunted House was the only one of them to ever speak.