The Royal Guards take the roll of keeping the castle under control and making sure suspicious characters do not harm it or its residents.
The guards are seen standing in rows of two while Cinderella walks down the red carpet to the ballroom. When midnight strikes and she flees the castle in her coach, the Grand Duke sends them on their horses to bring her back. However they returned and informed the Grand Duke that she vanished. They are last seen standing in rows of two while Cinderella and Prince Charming walk to their carriage during their wedding.
The guards open the front doors for Cinderella and Anatasia as they enter the palace. They see Jaq and Gus run into the palace while Lucifer chases them but they ignore their presence and go back to guarding the castle.
When Cinderella steals the Magic Wand back from Lady Tremaine, Lady Tremaine orders the guards to arrest her for stealing. Later when they try to confront Lady Tremaine at Prince Charming's wedding, she uses the wand to turn them into animals. It is unknown if the Fairy Godmother turns them back into their own selves or they change back automatically.