While Alice is chasing after the White Rabbit after being shrunk, she runs into a flower garden where she meets a large group of beautiful flowers. During Alice's attempt to befriend them, the children flowers exclaim they can sing, and they all want to sing about themselves, but the Red Rose (the leader) says they will sing "All in the Golden Afternoon" which is about all of themselves. After the song the flowers try to figure out what kind of flower Alice is. When Alice replies that she isn't a flower, they determine that she is a weed and change their attitude towards her, at which they chase her out of their garden violently. Alice tries to get her own back by telling them that she would pluck them all if she was her normal size, and two Daffodils respond by soaking her with water, sending her further away from the garden. They are not shown again for the rest of the film and cease to exist when Alice wakes up at the film's end.
Alice in Wonderland Jr.
In Alice in Wonderland Jr., there are only five flowers: Rose, Daisy, Lily, Violet, and Petunia. They make up a typical girl-group called "The Golden Afternoon" and deny Alice's request to join the club because she is a "silly little uncool weed."