Ploot has a two-sided nature, one that is benevolent and one that is chaotic. His initial appearance and helpful manner belies his true purpose and form: a giant polluting beast. In this form, he is very aggressive and dangerous, squirting pollution everywhere and harming those who get in his way, not even caring about the environment. However, after being sprayed with air freshener, Ploot's personality changed for the better, as he now cleans up the pollution and doesn't spit it out, helping to clean the environment as a result. He also appears to have gained control over his pollution form, since he turned into it to fight off Leroy clones with his cousins without an ill effect on his personality.
Experiment 505 was the 505th genetic experiment created by Jumba with Hämsterviel's funding. He was designed to collect pollution and trash, growing exponentially bigger as he hoarded more. When 505 collected enough, he would then convert the pollution into toxic sludge and spread it across the nearby area. 505 and the other first 624 experiments were deactivated and smuggled to Earth by Jumba during his mission to capture Experiment 626.
All of the experiment pods were released and scattered across the island of Kauai.
505's pod eventually made its way into a bag of trash, where it was thrown over a cliff by a couple of polluters into Pudge's underwater grotto, activating the experiment.
505, named Ploot, first began collecting all the pollution in the grotto, and then started collecting trash throughout town, but not before he was discovered by Lilo and Stitch while they were busy cleaning up the beach.
Unaware of what Ploot was really doing, Lilo and Stitch decided to let him carry out his primary function rather than attempt to stop him. However, Jumba later revealed Ploot's true intentions after Lilo informed him about the experiment.
Eventually, Ploot collected enough trash and pollution to grow into a monstrous form, and then began his rampage by spreading toxic sludge around the island of Kauai. Before long, most of Kokaua Town was covered in Ploot's toxic goo, which contaminated everything, including Pudge's grotto.
While an ailing Stitch was recuperating from an earlier garbage-eating incident (which Jumba theorized as a rare space disease), Lilo rallied several other experiments to try and stop Ploot from wrecking the environment. Despite their best efforts, Ploot was simply too strong for them.
Ploot eventually grabbed Lilo in one of his giant claws, ignoring her complaints, but she was saved by Stitch, who had fully recovered from his illness. Lilo then defeated Ploot by using natural air freshener (a product that Jumba and Pleakley discovered) to reduce the latter to his original size.
Ploot was then captured in a containment orb by Stitch, rehabilitated, and found a one true place cleaning up litter and pollution.
Later, the first 624 experiments, including Ploot, were rounded up by Leroy and taken to a stadium to be destroyed. However, Lilo, Stitch, Jumba, Pleakley, Reuben and Gantu arrived before the experiments could be destroyed.
Ploot participated in the following battle between the experiments and the Leroy clones.
The Leroys soon gained the upper hand in the battle, but were defeated when Lilo, Stitch, Reuben and several other experiments performed the song "Aloha ʻOe", which caused the Leroy army to shut down due to the original Leroy's fail-safe.
Ploot made an appearance in the Stitch! anime where he grew to his monstrous size and polluted everything again. This also caused a chain reaction since the island was somehow the center of Earth's power, and it nearly destroyed the planet.
- In his episode, Ploot is erroneously referred to as 515, a number taken by Deforestator who as well appears in that episode.
- The episode Ploot was in was aired on Earth Day.
- Despite Ploot's ability to metabolize trash and waste into toxic sludge, his weakness to the compounds in air fresheners is ironic; historically, aerosol-based air fresheners often use ozone-depleting chemicals, especially chlorofluorocarbons, as their main propellants, which are not the safest chemicals to the environment.
- Ploot's pod color is blue.
- Ploot is described by the experiment computer screen as, "Experiment 505. Primary function: Polluter."
- In Leroy & Stitch, Ploot was seen in his alternate pollution form during the battle between the Leroy army and the experiments.
- Ploot is one of the few experiments to not appear in the group photo at the end of Leroy & Stitch.