These mice wake up in the morning listening to Cinderella sing "A Dream is a Wish Your Heart Makes" as well as helping her dress up for the day. The mice later got some bits of corn for breakfast from Cinderella after Jaq distracts Lucifer so they could reach Cinderella in the garden.
Later, Cinderella shows them her mother's old dress, which she plans to redesign as her dress for the ball. When she is called away by her stepfamily for more work that keeps her occupied for the rest of the day, the mice, along with the birds, take over the duty of redesigning the dress (Cinderelly, Cinderelly), which they show to Cinderella at the end of the day.
Later, the mice watch in horror and dismay when the Stepsisters destroy Cinderella's dress, and when Cinderella ran out into the garden to weep, two of the mice, Bert and Luke, join Jaq and Gus on trying to console Cinderella. These four mice would later be turned into horses by the Fairy Godmother to take Cinderella to the ball. They return to their mouse forms after the clock strikes twelve at midnight.
The next day, after Lady Tremaine locks Cinderella in her room to prevent her from revealing herself as Prince Charming's true love, the mice and the birds fight Lucifer for the key, but they're no match for their mortal enemy. The battle finally ends in victory for them when Bruno arrives and chases Lucifer away. They later cheer for Cinderella when she reveals the other glass slipper to the Grand Duke, proving herself as Prince Charming's true love.
At the end of the film, the mice move into the palace and cheer for Cinderella's wedding with Prince Charming.
The mice help with the Fairy Godmother's stories that set the plot in motion. Jaq, Gus, and Mary had the most prominent role in this film, while the other mice were mainly seen in between each segment, with the exception of a few male mice appearing alongside them in the three shorts.
The mice make occasional appearances in House of Mouse alongside Jaq, Gus, and Cinderella.
- The mice do not appear in the third movie, Cinderella III: A Twist in Time, with the exception of Jaq and Gus. They were nevertheless alluded to in the opening when Cinderella asked the Prince what the mice were doing with her shoes.
- Out of all the mice in Cinderella other than Jaq and Gus, Suzy and Perla are the only ones to have made appearances at the Disney theme parks.