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"O Brother, There Art Thou" is the first segment of the fortieth episode of Yin Yang Yo! It premiered on May 5, 2008 alongside "Roger... Over and Out", and is the first segment of the fourteenth episode in season two.
When Yang separates himself from Yin and Master Yo, he saves a robot, and they start to act like brothers. But Yin is feeling suspicious and jealous and discovers the robot is actually a spy made by Zarnot sent to destroy Yang.
- The title is a reference to the film O Brother, Where Art Thou?