Mr. Jones is a pet iguana owned by Ron, the manager of Sleep Well. Ron trained him to retrieve toys from visitors and bring them to his office, where he placed them in a basket and rang a bell to alert Ron.
When Bonnie Anderson and her mother arrived at the hotel for the night, Mr. Jones, after a period of time, brought to Ron the entire toy gang along with Combat Carl, eating one of Mr. Potato Head's arms in the process. He then proceeded to rest, until he noticed Jessie sneaking toward the curtain hiding Ron's scheme. He attacked her, but after Jessie noticed Mr. Potato Head's arm and Combat Carl's hand (which Mr. Jones had eaten sometime before the main events of the story) in the back of Mr. Jones's mouth, she pushed her head and arm inside his mouth, retrieving the parts before escaping. With the help of Mr. Potato Head's arm, Jessie tricked Mr. Jones into yanking off Ron's curtain, revealing Ron's scheme to Bonnie and her mother. After Jessie froze because of presence of the humans, Mr. Jones retrieved her and brought her to the basket before ringing Ron's bell, further exposing his owner. Later, Ron fed Mr. Jones in a dog dish, which Mr. Jones proceeded to eat, just as two police officers arrive to arrest his owner for theft. It is unknown what happened to him afterwards.
- According to director Angus MacLane, Mr. Jones has a missing scale on his left breast, in allusion to the great dragon Smaug from Tolkien's The Hobbit. Incidentally, a film adaptation of The Hobbit featuring Smaug was released around the same time as Toy Story of Terror.
- He bears some resemblance to Daisy's pet iguana Knuckles from Quack Pack in both appearance and personality.
- He is also similar to Joanna the Goanna from The Rescuers Down Under, but is unlike her as well in that his playful attitude contrasts with Joanna's vengeful nature. In addition, unlike Joanna, his owner does not abuse him at all.
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