Millie and Melody Mouse are the nieces of Minnie Mouse, and the daughters of her sister, Mandie Mouse. Although they can be full of mischief, they are sweet just like their aunt. They are the female counterparts and arch-rivals of Mickey Mouse's nephews Morty and Ferdie Fieldmouse.
The only known speculative film appearance of either of Minnie's nieces is in 1983's Mickey's Christmas Carol, where Mickey Mouse, as Bob Cratchit, has a daughter. In this film Morty & Ferdie are said to have played Cratchit's two sons (including one as Tiny Tim), and since Melody seems to be the most consistent name used for any niece attributed to Minnie, it is quite likely that it was Melody who played the role of Bob Crachit's daughter.
In 2011, the twins would costar in the Disney Junior series, Minnie's Bow-Toons. They first appeared in the episode "Trouble Times Two" where they visit Minnie and are shown to be crafty troublemakers.
Mickey's Christmas Carol
In Minnie's Bow-Toons, they first appear in the episode Trouble Times Two. They've made semi-regular appearances since.
Mickey Mouse Clubhouse
In Mickey Mouse Clubhouse, they first appear in the special Minnie's Winter Bow Show.
Other characters: Figaro | Mortimer Mouse | Clara Cluck | José Carioca | Panchito Pistoles | Humphrey the Bear | J. Audubon Woodlore | The Phantom Blot | Morty and Ferdie Fieldmouse | Chief O'Hara | Millie and Melody Mouse | Butch the Bulldog | Yen Sid | Magic Brooms | Willie the Giant | Beagle Boys | Oswald the Lucky Rabbit | Ortensia | Amelia Fieldmouse | Gus Goose | Salty | Launchpad McQuack | Webby Vanderquack | Cuckoo Loca | Roxanne | P.J. | Bobby Zimmeruski | Stacey | Grandma Duck | Gladstone Gander | April, May and June