This special highlights many portions of Mickey Mouse's 50-year career, including his debut in Steamboat Willie, his color debut in The Band Concert, his feature debut in Fantasia, his foray into television with The Mickey Mouse Club and becoming the host of Disneyland and Walt Disney World. The special also explores Mickey's personal relationships, including his girlfriend Minnie Mouse and his arch-rival Peg-Leg Pete. Throughout the special, there is a three-part live-action/stop-motion short animated by and starring Mike Jitlov paying tribute to Mickey. The show closes with the special song "The Whole World Wants to Wish You Happy Birthday, Mickey Mouse".
Featured are many special guest stars, including Peter Sellers, Edgar Bergen, Annette Funicello, Kermit the Frog, Sally Field, O.J. Simpson, Karen and Richard Carpenter, and even former president Gerald Ford.
- The third part of the Mike Jitlov segment, Mouse Mania can be seen as a bonus feature on the Walt Disney Treasures DVD Mickey Mouse in Living Color: Volume 2.
- This special aired two months after the death of Edgar Bergen.