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All the Toon characters on this list have either appeared physically or in another form in Who Framed Roger Rabbit.
These characters had either appeared in animated features or cartoon shorts made by various studios, presented here.
Many additional characters were planned, or at least considered, for inclusion in the film but ultimately omitted due to legal issues and/or production limitations. Others managed to make it into the final film, but were cut out or replaced later on during production and development.
(*) Denotes anachronisms; these characters (or, in the cases of characters such as Tinker Bell and Marvin the Martian, the animated versions of them that appear in the film) were created after 1947. But as screenplay writer Peter S. Seaman said, "The aim was entertainment, not animation history." It also has been argued that the characters may have "existed" in the Toon world before being "discovered" and featured in real productions.