Lena Hyena is a hag-like toon woman voiced by June Foray in Who Framed Roger Rabbit. Eddie Valiant encounters her in Toontown, after, seeing her in silhouette in her apartment window, mistaking her for Jessica Rabbit due to their similar style of dress. Believing him to have come to her apartment out of romantic intent, she starts chasing him with love on her mind.

Originally named Lena the Hyena, she was created by Al Capp for his comic strip Lil' Abner in 1946. She was said to be the "Ugliest Woman in The World", so much so that anyone who saw her instantly went mad and no sane person was ever capable of describing her appearance.

Initially, Lena's face was usually hidden either behind censor bars and dialogue balloons or by being seen only from the neck down. Capp later decided to stop teasing his readers and invited them to draw the best interpretation of her in a nationwide contest. Notably, among the contestants was Carl Barks, the creator of Uncle Scrooge. The winning entry of was made by comic book artist Basil Wolverton, who would go on to do artwork for Mad Magazine.

She is portrayed in the Roger Rabbit comics as being a long-time friend of Jessica's.