Junior is very serious-minded and doesn't take kindly to trespassers, namely those who are not "cows," according to him. He talks in a deep, ominous voice and tries to maintain his composure. He also apparently has a crush on Mrs. Calloway, as he is seen complimenting her hat.
Junior is Alameda Slim's charming and gentlemanly "steed." Unusual for a villain's sidekick, he is portrayed as neither good nor bad, simply doing his job. He is very polite to cows and even flirts with Mrs. Calloway upon seeing her. Being a buffalo and not a cow, Junior is immune to Slim's yodeling. He also tires of Buck's arrogance when the horse attempts to get through the mine's entrance, despite Junior saying, "Cows only."
Maggie, Mrs. Calloway and Grace later show up along with Lucky Jack (a jackrabbit who helped the cows reach Echo Mine), and Junior lets them all pass. When Buck questions Junior about this, the buffalo states, "They're cows," and when Buck brings up the rabbit, Junior replies, "Well, obviously, he was with the cows." Buck tries convincing Junior that he was with the cows too, but to no avail.
Sometime later, when Buck gloats after tricking another horse into leaving his post so he could get back with Rico, Buck is overheard by Junior who snorts angrily, as he had been right behind him. Junior is later seen chasing Buck around Echo Mine. Junior is shown to be immensely strong and survives a considerable fall after the cows inadvertently push him down the mine shaft.
At the end of the movie, Junior has left the services of Alameda Slim (since Slim is in jail) and arrives at Little Patch of Heaven, courting Mrs. Calloway. He also dances with all the other farm animals as they celebrate Pearl's farm being saved and re-opened.
- When Junior gets pushed and falls down the mine shaft, his yell is supplied by the classic Goofy holler sound effect.
Characters: Maggie | Mrs. Calloway | Grace | Pearl Gesner | Sheriff Sam Brown | Buck | Rusty | Alameda Slim | The Willie Brothers | Rico | Mr. Wesley | Jeb | Audrey | Ollie | Piggies | Chicks | Larry | Lucky Jack | Patrick | Barry and Bob | Junior the Buffalo
Songs: (You Ain't) Home On The Range | Little Patch of Heaven | Yodel-Adle-Eedle-Idle-Oo | Will The Sun Ever Shine Again? | Magic Carpet Ride | Wherever The Trail May Lead | Anytime You Need A Friend | Heroes, Villains and Cows (deleted song)