Juju first appears in the film right after Mama Odie rescues Tiana and her friends from a pack of shadow demons. Juju is called by Mama Odie and shows his affections for her. It is shown she treats him like a baby. Juju is also shown during the scene to assist Mama Odie as her cane and guide through the tree due to her blindness. During the song Dig a Little Deeper, Juju is turned into a dog, pig, cow and a goat until returning to his original form. Juju appears at Tiana and Naveen's marriage and celebrates alongside Mama Odie and the bayou animals.