Very loyal and highly affectionate, Juju is Mama Odie's closest companion, acting as her eyes throughout their hidden home. And although Juju acts as Mama Odie's personal little assistant, he is also treated as her very own child, much like the stereotype that the elderly treat their pets as their own children.
Juju first appears in the film right after Mama Odie rescues Tiana and her friends from a pack of shadow demons. Juju is called by Mama Odie and shows his affections for her. It is shown she treats him like a baby. Juju is also shown during the scene to assist Mama Odie as her cane and guide through the tree due to her blindness. During the song "Dig a Little Deeper," Juju is turned into a dog, a pig, a cow and a goat before returning to his original form. Juju appears at Tiana and Naveen's marriage and celebrates alongside Mama Odie and the bayou animals.
- Juju is the only snake in a 2D Disney film who, unlike Kaa or other villainous snakes, is not an antagonist.
- Technically, the first snake who is a protagonist is Murgatroid from The Brave Little Toaster to the Rescue, the second one being Larry from The Wild.
- It should be noted the pythons seen in the Not One of Us sequence in The Lion King II: Simba's Pride technically count as good guys, albeit misguided.
- It is unknown what kind of snake Juju is. Although he appears to be some kind of constrictor snake, either a python or a boa.