She voiced Sergeant Calhoun in the 2012 Disney animated film Wreck-It Ralph and will reprise her role in its sequel. She also voiced Mrs. Johnson in three episodes of the Disney Channel animated series Phineas and Ferb, Jackie Greenway in Handy Manny, Queen Neptuna in the Toy Story Toons episode Small Fry, Joan Jameson in The Spectacular Spider-Man, and Mrs. Locks in Goldie and Bear.
According a November 2010 post by Thewrap.com, Jane Lynch was to appear in the film The Muppets as a prison guard. Lynch denied the rumour in a red-carpet interview with MaximoTV.com, but stated that she would enjoy doing so if offered. She also appeared in Girl Meets World as herself, Desperate Housewives as Maxine Bennett, Felicity as Professor Carnes, MDs as Aileen Poole, According to Jim as Janice, Monk as Dr. Julie Waterford, Criminal Minds as Dr. Diana Reid. She also appeared in the ABC TV show The Real O'Neals.