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"Game Over" is the first segment of the fifty-fourth episode of Yin Yang Yo! It premiered on October 27, 2008 alongside "Creeping with the Enemy", and is the first segment of the twenty-eighth episode in season two.
While Yo is gone, leaving Yin and Yang to look out for themselves, the Manotaur tries to demolish the dojo and build condos on the site. To prevent this, Dave wagers the Manotaur on a game of Wowza Wowza! If the Manotaur wins, not only do Yin and Yang have to give him their dojo, but their lives as well!