Each of the four girls wear the same thing, and they all have red hair. They are first seen at the town fountain, braiding each other's hair. When Flynn Rider brings Rapunzel to the city to see the floating lights, he whistles for the girls to help braid up Rapunzel's hair to make it easier to manage in the city, to their delight. After they finish braiding Rapunzel's hair, she happily thanks them.
At the end of the film, when the kingdom throws a big party to celebrate Rapunzel's returns, these four girls are seen holding and feeding Rapunzel's pet chameleon, Pascal. One of them gives Pascal back to their friend, Rapunzel.
The sisters also appear in the short sequel as attendees at Flynn (now going by his real name of Eugene Fitzherbert) and Rapunzel's wedding. One of the girls catches sight of Rapunzel in her wedding attire and becomes awestruck, causing her sisters and all other attendees to look at the bride. Along with everyone else, the four sisters celebrate as Rapunzel and Eugene finally tying the knot and sharing their first kiss as a married couple.