The cafè is where Tiana works to earn money for her restaurant, during the day. Employees, besides Tiana, include Buford, who ridicules Tiana about her dream. Charlotte La Bouff and her father, Eli "Big Daddy" La Bouff often stop by the restaurant to talk to Tiana. In the story, Doctor Facilier plans his evil plot here while pretending to order, and Tiana discovers that Prince Naveen, from Maldonia, will be visiting New Orleans.
In the video game, Duke's Cafè is the location for one of the mini-games, where the players must dance outside of the cafe without being caught by Buford.
- Menu items include hotcakes, eggs, and Tiana's popular beignets.