Dr. Calico (or simply "Calico," or sometimes "The Man With The Green Eye") is the main "antagonist" in the TV show that Bolt and Penny work in. Although he is a villanesque person, he isn't an actual villain, but merely an actor. Bolt, however, thinks he is a true villain, and is always promising to get him for real. Whenever Penny is not around, Bolt always thinks that Calico is the reason for her absence. After Bolt goes missing from the studio, they quickly find a replacement dog, however it got scared while shooting an episode and accidentally causes a fire. Calico, like the rest of the crew, survived the fire and continues his role as a villain in the show. He is voiced by Malcolm McDowell.
The character of Dr. Calico is among the most ruthless, cunning and merciless villains ever seen in the Disney universe. Always accompanied by one or more cats, he is seen as a master of planning and a brilliant strategist. Ruthless, yet patient, he likes to torture his enemies, in order to force them to do his biding. Although he is seen always hiding behind his minion guards, he is not above killing his enemies with his bare hands; this is seen in the show when he tries to kill Penny after she had surgery, by infiltrating the hospital, disguised as a doctor, in order to inject her with some kind of fatal drug. In the videogame, his personality seems more evil and sinister than in the film.
Aside from appearing in the film, Dr. Calico is also the major antagonist of the film's videogame adaptation, and he has appeared on the minisode Super Rhino, where Rhino takes on the part of hero, replacing Bolt.
In the videogame adaptation of the film, which tells us the adventures of Penny and Bolt in the television show, Dr. Calico's character is further developed, giving us a better perspective of what his main goals are, and where they operate.
After capturing Penny's father on the show, Calico then pursued the one thing that would get him to cooperate, Penny. Calico finally managed to capture Penny once she and Bolt found his temple base in the Ukatan jungle. To prove Penny was alive and in custody to her father, Calico gave Penny a cell phone with her father on the other line. Calico then attempted to flee the crumbling temple with his new captive, but decided to leave her there with Bolt to die, saying, "As long as the professor believes she's alive, he will do what is asked and finish the weapon. Too bad we can't all have nine lives."
However, Penny and Bolt survived and discovered Calico's new arctic base with a large rocket in the center. But upon entering, Penny notices micro-focusing mirrors and realizes that it is her father's satellite instead of a warhead. All too late as Calico seals the entrance and, to Bolt's horror, launches it with Penny inside saying. Bolt then jumped onto the rocket and dismantled it in mid-flight, crashing it into the snow.
The two then ventured to Calico's harbor in Japan, where they discovered a ship full warheads. Just then, Calico arrived at the harbor, enraged to find Penny still alive, and even more so as he watched his prized freight ship sink into the water, thanks to Bolt, who then pursues Calico, escaping in his car to a nearby train station. He arrives at the station and boards his train, closely followed by Penny and her dog. Just as the duo finally corners the doctor, he slips into a hidden room and escaped on his private jet. Not before Penny secures a homing beacon onto the hull, leading them right back to Calico's base.
"Are you crying? Is it because I kidnapped you daddy? Or because I hurt your precious little dog? Then...we're even! Because you and your little mutt...you sunk my ship, destroyed my cars, thrashed my men, and..'.actually, your sad little displays don't affect me." -Calico to his captive, Penny
Penny and Bolt traced his jet back to his main base, where Penny's father was being held. However, upon arriving, Calico used his new weapon on Bolt, draining him of his powers and strength and capturing Penny. Once Penny is inside, he explains his plan to use Penny's father's micro-focusing mirror-equipped satellites to bounce a beam around the Earth and neutralize and destroy all atomic weaponry execpt for his own. And as he leaves, he violently kicks Bolt's cage proving Penny of her limited knowledge. However, during this, Penny maneuvered her way out of her hand bindings.
Once free, she attempted to find her father to save Bolt. Unfortunately, she is only captured by the doctor once again, just as Bolt regains his powers and Penny sabotages the control panel, however, raising the bridge so Bolt could reach her. Once he did, Penny escaped from her guard captors yet again and launched a pre-prepared, rocket powered cart at the main power generator (which Bolt exposed). Then, using what power remained in the generator, Calico dueled with Bolt, using powerful lightning bursts. But after the lengthy and hard-fought battle, Bolt finally defeated him. Beaten and weakened, he attempted to escape on his private helicopter, launching numerous missiles as a decoy, just as Penny finally finds her father (aboard the helicopter). But while Bolt tried to apprehend Calico by grabbing his foot, Penny's father pleads to her and Bolt to allow Calico to escape with himself to stop the missiles, reminding her that she had the power to find him again. Regretfully, Penny ordered Bolt to stop the missiles as Calico escaped with her father.
- Dr. Calico is one of the few Disney Villains who is not evil due to fact that he is simply an actor portraying the part of the villain in the Bolt televison show. This also makes him the very first Disney Villain to not have any genuine hatred towards or a vendetta against the film's heroes. The second being the The Backson who mistakenly thought for being evil.
- Although he is called Dr. Calico, it is never really explained what kind of doctor he may be. It is implied that aside from being the leader of a major terrorist organization, he could also be a medical doctor, or a scientist of genetics and/or robotics.
- According to Rhino, at one point Dr. Calico had a plan to infiltrate the Olympics with gymnastic cyborgs; A plan which was foiled by Bolt.
- When creating Dr. Calico, the film's development team modelled his appearence and personality after some of James Bond's most famous villains such as Dr. No, Auric Goldfinger, Emil Stravos Blofeld, Aristotle Kristatos, Hugo Drax, Brad Whitaker and Francisco Scaramanga.
- Dr. Calico is one of the few villains to not have a musical theme or song of his own, unlike such villains as Captain Hook, Jafar, Scar, Judge Claude Frollo, Oogie Boogie, Ursula, and Dr. Facilier. This could be possibly be attributed to the fact that he is not a true villian and that the above characters are all featured in musicals, whereas Bolt is not a musical.