For Disney, Trejo filmed a cameo for the 2011 film The Muppets as a prisoner; however, his scene was cut from the final theatrical film. Footage from the scene was shown in The Fuzzy Pack trailer. He did, however, have a role in Muppets Most Wanted where he plays a Gulag prisoner (also named Danny Trejo; Trejo was an inmate at San Quentin State Prison for 3 years).
His other Disney roles include Ox in the The Spectacular Spider-Man episode "Probable Cause", Johnny-23 in the 1997 Touchstone Pictures film Con Air, Venom in Phineas and Ferb: Mission Marvel, Antonio Agama in Elena of Avalor, Wreck Marauder in Tangled: The Series, Vasquez in Big City Greens and the lead piñata in the Mickey Mouse episode "¡Feliz Cumpleaños!".