The Bunny Girls are characters from the 1935 Silly Symphony, The Tortoise and the Hare and Toby Tortoise Returns. They attend Mrs. Cottontail's Girl's School and cheer on Max Hare at whatever he does such as racing and boxing.
The Tortoise and the Hare
After Max Hare pretends to sleep he runs until he find a school of girls. When he starts to notice them, they begin to swoon over him. When Toby Tortoise walks by they ask him to play with them he replies he has a race to finish. Max Hare shows them archery tricks and shows how fast he is by playing tennis by himself, running back and forth hitting the ball. The Bunny Girls clap and cheer for him and swoon some more. Max gets back to the race and loses. They are not shown again.
Toby Tortoise Returns
There are only three Bunny Girls in Toby Tortoise Returns. They attended the boxing match to cheer on Max Hare. After Max Hare dodges Toby's punches he turns to the girls who begin to giggle. When Toby tries to sneak up on Max, they warn him, but he already knows and punches Toby.