Unlike the character's depiction in the earlier illustrations of Frederick S. Church, A. B. Frost and E. W. Kemble, the Disney animators redesigned Br'er Bear in a more slapstick, cartoony style. Br'er Bear is also prominently featured in the Disney theme park log flume ride Splash Mountain. The plot for Splash Mountain was based entirely on the Uncle Remus stories.
Br'er Bear is portrayed as stupid, slow-witted, prone to violence when provoked and gullible, leaving him open to being tricked repeatedly by Br'er Rabbit when he accompanies Br'er Fox (due to ignoring the latter's warnings of Brer Rabbit's many tricks).
B'rer Bear is the secondary antagonist in Song of the South. He first appears in the 1st animated segment "B'rer Rabbit runs away". He is introduced when Uncle Remus says" When he hears ol' B'rer Bear ambling' down the road". He then is tricked by B'rer Rabbit into getting himself tied up in B'rer Fox's trap for B'rer Rabbit. When B'rer Fox finds B'rer Bear tied up, he cuts B'rer Bear down. B'rer Bear then gets into an argument with B'rer Fox claiming "I was making a dolla' a minute!" When B'rer Fox convinces him it was a trick, B'rer Bear begins to march towards B'rer Rabbit claiming " I'm just gonna knock his head clean OFF". He and B'rer Fox both lunge at B'rer Rabbit, who escapes the fight, leaving Brer Fox to get repeatedly and inadvertently beaten by Brer Bear.
B'rer Bear helps to create the Tar Baby in segment 2. He is sitting on a log while B'rer Fox builds the baby. B'rer Fox then comments that all it needs are eyes and rips the button off B'rer Bear's coat. B'rer Fox then rips the bowl off B'rer Bear's pipe to create a nose for the tar baby. B'rer Fox then rips hair off B'rer Bear's butt to give the baby some hair. They then carried the baby out into the woods on a log. B'rer Rabbit then gets caught in the tar. When B'rer Fox and B'rer Bear retrieve B'rer Rabbit, B'rer Fox wants to cook him, but B'rer Bear just wants to "knock his head clean OFF". B'rer Bear then swings widely at B'rer Rabbit and B'rer Fox, causing B'rer Fox to toss B'rer Rabbit into the Briar Patch. When B'rer Rabbit acts like he is dead, B'rer Fox and B'rer Bear remove their hats in respect. When B'rer pops up, B'rer Bear softly hits B'rer Fox in the head in disappointment before leaving him.
In segment 3, B'rer Bear and B'rer Fox have finally captured B'rer Rabbit. B'rer Fox is preparing to roast B'rer Rabbit as B'rer Bear sits at the table. When B'rer Rabbit burst out laughing and tells B'rer Fox about his laughing place, B'rer bear wants to see this laughing place. The 3 B'rers then walk through the forest, B'rer Rabbit and B'rer Bear sing "Everybody's got a Laughing' Place". B'rer Bear dives into the beehive declaring " me first, me first", because he thinks B'rer Fox will try to beat him into the laughing place. B'rer Bear is then heard yelling in pain and emerging with a beehive on his nose declaring " There's nothing in here except BEES!". When B'rer Fox laughs at him, B'rer Bear and B'rer Fox are then chased around by bees, then chased away by bees with B'rer Rabbit laughing.
Br'er Bear can be spotted in several scenes of the film with other toons and at the very end of the film.
Br'er Bear made a cameo appearance in the episode "Casabonkers" during the Rubber Room Song. He is seen trying to make a hot dog.
Br'er Bear makes cameo appearances in several episodes of the House of Mouse TV series and in the direct-to-video release Mickey's Magical Christmas: Snowed in at the House of Mouse.
Brer Bear's silhouette can be seen at the end of the film with several other characters in the theater.
Like many of the Disney characters from movies, Br'er Bear appeared in the Disney comics. Usually he was with his castmates from Song of the South, being tricked by Br'er Rabbit. Sometimes Br'er Fox would be the one to try and bilk Br'er Bear out of something, such as his chicken herd. This would often be a mistake for Br'er Fox as Br'er Bear would catch on at the last minute and proceed to threaten him. Disney comics were also known for juxtaposing characters from different movies and shows, and Br'er Bear was one such case where he was hunting Bambi and his forest friends. Br'er Bear comes across his fellow villainess the Witch, who is mixing a cauldron. When Br'er Bear demands to know what the "magic potion" is, she tells him it is nothing special and it is best not to be concerned. However, Br'er Bear believes she is mixing a potion for super strength, and that she should not be hogging it, so he helps himself to some of it. When Br'er Bear blanches in disgust at how awful the formula tastes, the Witch angrily says all she was doing was making ordinary turpentine, and that is why she warned him not to drink any. Br'er Bear is then too weakened and humiliated to continue hunting Bambi, and Bambi and his forest friends rejoice that they are safe for now and that Br'er Bear was his own worst enemy due to his arrogance and refusal to heed warnings.
Br'er Bear appears in the game as a meet-and-greet character at the entrance to Critter Country, between The Haunted Mansion and Splash Mountain. Br'er Bear also joins Br'er Fox in the Splash Mountain mini-game. Br'er Rabbit and the player pay a visit to Br'er Fox's house, where Br'er Rabbit tricks his foes into believing that he tripped and fell down and is now helpless in front of their door. Br'er Bear and Br'er Fox rush out only to find a "present". Br'er Bear eagerly opens it to discover a beehive, although he doesn't realize it's a hive until the bees come out and attack. Br'er Bear accompanies Br'er Fox in chasing after Br'er Rabbit and the player, but they are ultimately foiled.
Br'er Bear appears as a walk-around character in addition to his appearance in Splash Mountain.
Br'er Bear plays his usual role as Br'er Fox's bumbling sidekick, giving Br'er Fox a boost to see Br'er Rabbit planning to leave home, getting caught in a rope trap, being stung by bees in the Laughing Place and in the end being caught in the Briar Patch while happily singing Zip-A-Dee-Doo-Dah.
Br'er Bear, Br'er Fox and Br'er Rabbit appeared near the end of the show to sing Zip-A-Dee-Doo-Dah.