In the film, Uncle Remus tell tales of Br'er Rabbit and his enemies Br'er Fox and Br'er Bear. Br'er Rabbit lives out most of his roles in the story book.
In one of the stories, Br'er Fox and Br'er Bear attempt to use the Tar Baby to capture and eat Br'er Rabbit. Br'er Rabbits gets stuck inside, but eventually escapes by Br'er Fox throwing him into a briar patch which ends in peculiar results.
In one of the other stories. Br'er Rabbit is captured by Br'er Fox and Br'er Bear and nearly eaten until he tricks them into taking him to his "Laughing Place".
Br'er Rabbit makes numerous cameo appearances in the animated series House of Mouse.
His most notable cameo is in the first episode "The Stolen Cartoons", where Mickey Mouse reminds everyone of the House of Mouse rules. As one of the rules, Mickey says "No villainous schemes", at which point Br'er Fox and Br'er Bear stop themselves from catching Br'er Rabbit.
Br'er Rabbit appears in the game as an unlockable meet-and-greet character outside the entrance to Splash Mountain in Critter Country, after the player helps Br'er Fox lure him out of hiding.
Br'er Rabbit also serves as the player's guide in the Splash Mountain mini-game. Br'er Rabbit and the player pay a visit to Br'er Fox's house, where Br'er Rabbit tricks his foes into believing that he tripped and fell down and is now helpless in front of their door. Br'er Fox and Br'er Bear rush out only to find a "present". Ignoring Br'er Fox's warnings that it's most likely one of the rabbit's tricks, Br'er Bear eagerly opens it to discover a beehive, although he doesn't realize it's a hive until the bees come out and attack. Br'er Fox and Br'er Bear then start chasing after Br'er Rabbit and the player, but the two villains are ultimately foiled.