She was mentioned in the Austin & Ally episode "Costumes & Courage", the The Muppets episodes "Got Silk?" and "Generally Inhospitable", the Stuck in the Middle episodes "Stuck in the Sweet Seat" and "Stuck with a Boy Genius", an episode of Lab Rats: Elite Force, an episode of Gamer's Guide to Pretty Much Everything, Doctor Strange (with her song "Single Ladies (Put a Ring on It)" heard), the BUNK'D episode "Cabin vs. Cabin", the Raven's Home episode "You're Gonna Get It", the MECH-X4 episode Versus The Outbreak, the Once Upon a Time episode "Secret Garden" and the K.C. Undercover episode "Welcome to the Jungle". She will voice Nala in the upcoming 2019 film, The Lion King.
- Knowles was one of various African-American celebrities to be considered for the role of Tiana in the movie The Princess and the Frog. Other candidates included Tyra Banks and Jennifer Hudson.
- A parody of Crazy in Love can be heard in the That's So Raven episode "Hizzouse Party".