She was mentioned in the Austin & Ally episode "Costumes & Courage", the The Muppets episodes "Got Silk?" and "Generally Inhospitable", the Stuck in the Middle episodes "Stuck in the Sweet Seat" and "Stuck with a Boy Genius", an episode of Lab Rats: Elite Force, an episode of Gamer's Guide to Pretty Much Everything, Doctor Strange (with her song "Single Ladies (Put a Ring on It)" heard), the BUNK'D episode "Cabin vs. Cabin" and the K.C. Undercover episode "Welcome to the Jungle".
- Knowles was one of various female, African American celebrities to be considered for the part of Tiana in the movie The Princess and the Frog. Other candidates included Tyra Banks and Jennifer Hudson.