She was mentioned in the Austin & Ally episode "Costumes & Courage", the The Muppets episodes "Got Silk?" and "Generally Inhospitable", the Stuck in the Middle episodes "Stuck in the Sweet Seat" and "Stuck with a Boy Genius", an episode of Lab Rats: Elite Force, an episode of Gamer's Guide to Pretty Much Everything, Doctor Strange (with her song "Single Ladies (Put a Ring on It)" heard), the BUNK'D episode "Cabin vs. Cabin", the Raven's Home episode "You're Gonna Get It", the MECH-X4 episode Versus The Outbreak, the Once Upon a Time episode "Secret Garden" and the K.C. Undercover episode "Welcome to the Jungle". She will voice Nala in the upcoming 2019 film, The Lion King.
- Knowles was one of various African American celebrities to be considered for the role of Tiana in the movie The Princess and the Frog. Other candidates included Tyra Banks and Jennifer Hudson.
- A parody of Crazy in Love can be heard in the That's So Raven episode "Hizzouse Party".