Bambi's mother is shown during the first of the film, having just given birth to Bambi, her only son. She is often teaching Bambi about the forest and warning him of the dangers of Man. She and Bambi endure Summer, and then a harsh Winter together in which there is little food in the forest. When out in the meadow, she and Bambi find a small patch of grass to graze, suggesting that Spring was near.
Halfway through their meal, she senses danger, and told Bambi to run back to the den as fast as he could. She sprints after him, continuing to urge him onward. Gunshots are heard in the background, but she tells him to keep running, and not to look back. After a loud gunshot rings throughout the forest, Bambi makes it safely back to the den. He turns, expecting her to be with him, only to find silence.
He leaves the den and begins calling for her, but she does not answer. The Great Prince finds Bambi, and tells him that she could no longer be with him, and then tells him to come with him. This reveals the death of Bambi's mother, and that The Great Prince was his father. She had sacrificed herself to save Bambi and the Great Prince.
Bambi's mother makes a short appearance to Bambi in his dream, telling him that everything would be alright, and that she would always be with him in his heart. After waking up, Bambi still hears her voice. He goes to the meadow and discovers she is not there. Her voice still trails out on the meadow sending Bambi to find her and becomes in a life and death situation. After being chased by Man's dogs, he asks his father if she is ever coming back, and the Great Prince answers no, meaning that Bambi's mother is gone, but probably dead.
- At one point, the film, Bambi, was meant to show her body after she was shot, but was later removed as it would be too dark.
- In The Sword in the Stone, Kay is hunting a deer who looks very much like Bambi's mother.
- In The Jungle Book, a deer that strongly resembles her is grazing in a meadow, and being unknowingly stalked by Shere Khan.
- In The Rescuers, during the song "Someone's Waiting For You", she and Bambi are seen eating grass in Devil's Bayou.
- She is similar to Tod's mother in The Fox and the Hound and Mufasa in The Lion King in the sense of teaching her son the lessons of life and death at her son's young age. Both are also killed by the film's main villain (Mufasa by Scar and Bambi's mother by Man). Both also appear in their son's dream/nightmare in the second films (Mufasa in sequel and Bambi's mother in midquel).
- She, along with many other Disney Characters, made cameo appearances in the film, Who Framed Roger Rabbit.
- In Beauty and the Beast, there is a deer grazing in the opening scene that looked very much like her.
- The Toy Story Toons "Small Fry" character Tae-Kwon Doe's head resembles Bambi's mother.
- A deer resembling Bambi's Mother appears in Sofia the First, episode "Holiday in Enchancia", as one of the forest animals who help Sofia track down her father.
Characters: Bambi | Thumper | Flower | Faline | Geno and Gurri | Friend Owl | Great Prince of the Forest | Bambi's mother | Aunt Ena | Thumper's sisters | Miss Bunny | Bluebelle | Man | Hunter Dogs | Ronno | Ronno's mother | Mrs. Quail | Pheasant | Young Bucks | Possums | Porcupine | Mrs. Hare | Mena