Ena appears when she is with Bambi's mother out in the meadow when Bambi hides behind his mother when being chased by Faline. Faline says to Ena that Bambi is bashful to which Ena replies that he may not be if Faline says hello to him. Bambi later chases Faline around Ena and his mother before the two fawns run further into the meadow together whilst being watched on by their mothers.
Ena, Faline, Bambi, Bambi's mother along with the other deer leave the meadow after being alerted of Man's arrival by Bambi's father The Great Prince of the Forest.
At the end of the film, Ena gains two grandchildren named Geno and Gurri.
In the original novel, of which Bambi is based on, Ena is actually Bambi's maternal aunt.
In the book, she's the mate of an unknown buck, and also the mother of Faline's twin brother, Gobo, who would later fall in love and become mates with a young doe named Marena who, as a result, becomes her daughter-in-law.
In the book's sequel Bambi's Children, she gains two adoptive grandchildren when Faline adopts the two orphaned bucks, Mambo and Nello.
Both are the mother of the protagonist's love interest (Faline and Nala)
Both are a friend of the protagonist's mother (Bambi's mother and Sarabi)
Both at one point where sisters with the protagonist's mother (Ena was sisters with Bambi's mother in the original book whilst Sarafina (then called Naanda) was sisters with Sarabi in the original script to The Lion King.