One can recognize the girls in their modern look: April (Lizzy in Dutch comics) has one ponytail in her hair and wears red, May (Juultje in Dutch comics) wears a headband, and dresses in blue, and June (Babetje in Dutch comics) has the two pigtails, and wears yellow or green.
To date, the girls' only appearance in animation was a cameo in the House of Mouse episode "Ladies' Night", where they take over for Huey, Dewey, and Louie as the club's band.
The first appearance of the trio was in the Carl Barks comic story Flip Decision, in which Donald is making all of his decisions based on coin toss. They can be seen when Daisy is visiting her sister, who remained unseen. In the final panel, they appear with Huey, Dewey, and Louie as their escorts.
It would be six years before they would be used in comics again. Their second appearance was in The Double Date, again with the nephews in the last panel.
The nieces also made an appearance in A Sticky Situation, in which they go on a dangerous trip with their aunt Daisy in order to flee the men in Duckburg. This comic marked the first time they were given an important role in the storyline.
They made their third appearance in the one-pager Small Fryers. When they get bored while visiting their aunt Daisy, Daisy agrees on letting them bake cookies by themselves (with disastrous results).
In their second to last appearance Movie Mad, Huey, Dewey, and Louie are soaking wet and run away to find shelter in a house where they unexpectedly meet the girls. They jokingly give the boys some dry clothes that happen to be dresses.
Their final appearance in Barks' comics was in The Beauty Business, which marks their most important role as they help Donald with his failing beauty salon in which Daisy has to go and try out. The girls fail at their jobs at first, but in the end, they give Daisy a perfect face mask and all is well.
In 1998, the editors of the Dutch 'Donald Duck' weekly magazine decided the three girls should be modernized, and they got permission from the Disney Company to do so. Dutch Duck comic artist Mau Heymans designed a new hairdo and new wardrobe for April, May, and June.
The new hairdo first appeared in the Dutch comic "Nieuwe Coupe". They now don't look the same at all in Dutch stories and don't have the 'girly' occupations anymore they had when Barks created them. In some Danish stories, the new hairdo has been copied. The Dutch comics with the redesigned girls have also been published in other countries. However. They still appear in their old design in several other countries that haven't adopted this redesign yet.
While April, May, and June still appear regularly in comics, their roles are usually quite minor. The exception is the Dutch version of Donald Duck comics, in which they appear quite often. They mostly appear in the Dutch series "Duckies", which focuses on April, May, and June, Huey, Dewey, and Louie and several classmates and teachers, who were designed by Daniel Branca at the request of the Dutch editors.
In Italian comics, they are called Ely, Emy, and Evy. Although the Italian illustrators have not adapted to the new designs of Daisy's nieces, Italian is one of the very few countries that use the trio frequently.
April, May, and June make a brief cameo appearance in the four-part crossover conclusion of the 2011 Darkwing Duck and DuckTales comics Dangerous Currency. They help the fellow residents of Duckburg beat back the villains and their slime.
- When Don Rosa was asked if the mother of April, May, and June could possibly be Donna Duck, who appeared in a few stories before Daisy appeared, he replied that he found it a good idea. Although this is, however, proven to be impossible, as Daisy and Donna were told to have never seen each other before their meeting in Al Taliaferro's 1951 newspaper strips.
- "Ladies' Night" shows April (wearing red) as left-handed.