Anne Seymour (September 11, 1909 - December 8, 1988) was an American film and television character actress.

Her Disney roles included Mrs. Amelia Tarbell in Pollyanna, Maggie Hargrove in the two-part Wonderful World of Disney episode "The Wacky Zoo of Morgan City", and Aunt Winifred in the Disney Sunday Movie episode "The Leftovers."