She is a former cleaning lady who worked at a research lab. One day she accidentally inhaled the fumes of an experimental bathroom disinfectant. The fumes had a strange effect on her and turned her into a cleaning-obsessed villain. She was later recruited by F.O.W.L. for their dastardly plans. She hates, and is even afraid of, dirt. She has a sister named Ample Grime, also a F.O.W.L. agent.
Ammonia Pine appears as the primary villain in only one episode--"Dirty Money" (the 3rd episode in production order). Her other two appearances, "Cleanliness is Next to Badliness" and "Dirtysomething", feature her paired with Steelbeak and Ample Grime respectively. She also makes cameo appearances in "Ghoul of My Dreams", "In Like Blunt" and "Time and Punishment".
Ammonia Pine interestingly never appeared in any of the Darkwing Duck comics published when the show was originally airing. She finally appeared in the comics in the form of a brief cameo in "Crisis on Infinite Darkwings", the second arc of the Darkwing Duck comic by Boom! Studios. She also appears alongside fellow F.O.W.L. agents Hotshot and Flygirl in "F.O.W.L. Disposition".
Ammonia reappears in the DuckTales/Darkwing Duck crossover story "Dangerous Currency" where she is a member of the "League of Eve-il" along with Magica De Spell, Cinnamon Teal and Camille Chameleon. They are in an alliance with the Phantom Blot, the Fearsome Four, and the Beagle Boys. They are also later joined by Negaduck. However, the crossover is being retconned as non-canon in the comic's continuation by Joe Books, so Ammona's whereabouts following "F.O.W.L. Disposition" are officially unknown as of this writing.
Heroes and Allies: Darkwing Duck | Launchpad McQuack | Gosalyn Mallard | Honker Muddlefoot | Tank Muddlefoot | Herb and Binkie Muddlefoot | Gizmoduck | Morgana McCawber | Neptunia | Stegmutt | J. Gander Hooter | Derek Blunt | Vladimir Goudenov Grizzlikof | Comet Guy
Villains: Negaduck/Negatron | Bushroot | Megavolt | Liquidator | Quackerjack | Steelbeak | Taurus Bulba | Hammerhead Hannigan | Hoof and Mouth | Tantalus | Clovis | Tuskernini | Dr. Fossil | Phineas Sharp | Professor Moliarty | Ammonia Pine | Paddywhack | Lilliput Gooney | Jambalaya Jake | Gumbo | Bug Master | High Command | Major Synapse | Hotshot and Flygirl | Splatter Phoenix | Camille Chameleon | Anna Matronic | Isis Vanderchill | Johnny T. Rex | Fluffy | Dark Warrior Duck