The year 2000 (MM) was a common leap year that started on a Saturday.
- Walt Disney Pictures introduced a different logo, which for the most part, only appeared on live-action films, with the exception of Dinosaur, Brother Bear, and the Platinum Edition of The Lion King.
- DiC's contract with The Walt Disney Company ends, though some of their shows, such as Madeline and Sabrina: The Animated Series would continue to air on various Disney-owned channels until 2004. DiC was later acquired by and folded into Cookie Jar Group in 2008.
- February 16 - Disneyland celebrates its 45th anniversary.
- February 26 - Disney holds a wrap party for Recess (and its film, Recess: School's Out), having completely finished production. The remaining episodes would continue to air from February 27, 2000 to November 5, 2001; the movie premiered on January 10, 2001 and was widely released February 16, 2001.
- November 12 - Mary Poppins premieres at the Wisconsin International Children's Film Festival.
- January 1 - Fantasia 2000 was released in IMAX theaters.
- February 4 - Gun Shy (Hollywood Pictures)
- February 11 - The Tigger Movie was released to favorable reviews and becomes a box office success.
- March 10 - Mission to Mars (Touchstone Pictures)
- May 19 - Dinosaur was released to mixed reviews but becomes a box office success.
- June 9 - Gone in 60 Seconds (Touchstone Pictures)
- June 16 - Fantasia 2000 was released theatrically worldwide.
- July 7 - Disney's The Kid and Scary Movie
- September 15 - Duets (Hollywood Pictures)
- September 29 - Remember the Titans was released to very positive reviews and becomes a box office success.
- October 13 - Whispers: An Elephant's Tale
- November 22 -
- December 15 - The Emperor's New Groove was released to very positive reviews, but only moderate box office success.
Home video releases
VHS & DVD releases
- January 11 - Toy Story (VHS only), Winnie the Pooh: A Valentine for You, and Inspector Gadget: Gadget's Greatest Gadgets
- January 18 - Safety Patrol, Sister Act, Sister Act 2: Back in the Habit, and Adventures in Babysitting
- February 1 - Tarzan, Mulan, and Pretty Woman: 10th Anniversary Edition
- February 15 - When a Man Loves a Woman
- March 7 - Pinocchio
- March 28 - The Sixth Sense
- April 4 - The Aristocats
- April 18 - Tarzan: Collector's Edition
- May 2 - The Fox and the Hound, The Three Caballeros, and Saludos Amigos
- May 9 - Father of the Bride Part II
- May 30 - Geppetto and Life-Size
- June 6 - Pocahontas, Make Mine Music, and Melody Time
- June 13 - Blood In Blood Out
- June 20 - Fun and Fancy Free and A Goofy Movie
- July 4 - Alice in Wonderland, Mary Poppins, and Robin Hood
- July 11 - Winnie the Pooh and the Honey Tree, Winnie the Pooh and the Blustery Day, Winnie the Pooh and Tigger Too!, Winnie the Pooh and a Day for Eeyore, and Sailor Moon: The Doom Tree Collection (final VHS release by Buena Vista Home Entertainment before ADV began licensing Sailor Moon)
- August 1 - Hercules, The Rescuers Down Under, and A Bug's Life
- August 8 - Reindeer Games
- August 22 - The Tigger Movie
- September 12 - Mission to Mars (DVD only)
- October 3 - The Black Cauldron and The Adventures of Ichabod and Mr. Toad
- October 10 - Shanghai Noon (Touchstone Home Video)
- October 17 - Beowulf (Dimension Home Video), Toy Story (DVD only) and Toy Story 2
- November 7 - Mickey's Once Upon a Christmas
- November 14 - Fantasia 2000 and Fantasia: Special 60th Anniversary Edition
- November 21 - Smart House
- December 12 - Scary Movie
- January 18 - From Dusk Till Dawn 3: The Hangman's Daughter (Dimension Home Video)
- February 29 - An Extremely Goofy Movie
- March 7 - Sing a Song with Tigger
- March 14 - The Prophecy 3: The Ascent (Dimension Home Video)
- June 13 - Discover Spot
- June 14 - The Crow: Salvation (Dimension Home Video)
- July 18 - Crime Story (Dimension Home Video)
- August 8 - Buzz Lightyear of Star Command: The Adventure Begins
- September 5 - Pocahontas II: Journey to a New World
- September 19 - The Little Mermaid II: Return to the Sea
- October 3 - Hellraiser: Inferno (Dimension Home Video)
- December 12 - Air Bud: World Pup
- January 11 - Rolie Polie Olie Season 2 final episode premieres on Playhouse Disney.
- February 26 - The Weekenders premieres on ABC.
- June 17 - Even Stevens premieres on the Disney Channel.
- August 30 - Rolie Polie Olie Season 3 premieres on Playhouse Disney.
- September 9 - Teacher's Pet premieres on ABC.
- September 27 - PB&J Otter premieres its final episode on Playhouse Disney.
- October 2 - Buzz Lightyear of Star Command premieres on ABC and in syndication.
- March 23 - Walt Disney World Quest: Magical Racing Tour
- June 7 - Winnie The Pooh And The Blustery Day: Activity Center
- September 13 - The Little Mermaid II: Return to the Sea
- September 24 - The Little Mermaid II: Pinball Frenzy (North America only)
- September 29 - Tigger's Honey Hunt
- October 4 - Alice in Wonderland
- October 19 - Donald Duck: Goin' Quackers!
- November 13 - Mickey's Speedway USA for Nintendo 64
- November 15 - 102 Dalmatians: Puppies to the Rescue for PC, PlayStation, and Game Boy Color and 102 Dalmatians: Activity Center for PC
- November 24 - Dinosaur for Sega Dreamcast
- November 30 - Dance Dance Revolution Disney Dancing Museum for Nintendo 64 (Japan only) and Dance Dance Revolution Disney Mix for PlayStation and arcade
- December 1 - Disney's Aladdin in Nasira's Revenge for PlayStation and Mickey's Speedway USA for Nintendo 64 (Europe only)
- December 15 - Doug's Big Game for Game Boy Color
- December 29 - The Lion King: Simba's Mighty Adventure for Game Boy Color
Theme Park Happenings
- June 25 - 23-year-old Cristina Moreno suffers a brain hemorrhage after riding Indiana Jones Adventure: Temple of the Forbidden Eye at Disneyland.
- July 20 - Disney Ambassador Hotel opens at the Tokyo Disney Resort.
- September 4 - Pooh's Hunny Hunt opens at Tokyo Disneyland.
- September 22 - 4-year-old Brandon Zucker suffers brain damage after falling out and being dragged by a car on Roger Rabbit's Car Toon Spin at Disneyland and later dies in 2009 at the age of 13.
- September 25 - Rocket Rods closes at Disneyland for "refurbishment", with a projected Spring 2001 reopening; it never reopened.
- April 2 - Odella Otter
- August 8 - XR, Mira Nova, Booster, Commander Nebula, Warp Darkmatter
- December 15 - Kuzco, Pacha, Chicha, Yzma, Kronk, Incan Palace Guards, Bucky the Squirrel, Chaca, Tipo, Yupi
- January 8 - Noah Cyrus (actress and singer)
- January 26 - Piper Mackenzie Harris (child actress)
- February 5 - Jordan Nagai (child actor)
- March 6 - Jacob Bertrand (actor)
- March 21 - Jace Norman (actor)
- April 3 - Ella Bleu Travolta (actress)
- April 6 - CJ Adams (actor)
- May 30 - Jared S. Gilmore (actor)
- June 21 - Natalie Alyn Lind (actress)
- July 16 - Jonathan Morgan Heit (actor)
- July 25 - Mason Cook (teen actor)
- July 28 - Emily Hahn (child actress)
- August 3 - Landry Bender (actress)
- August 16 - Cole Jensen (actor)
- September 28 - Frankie Jonas (actor and singer)
- October 6 - Isobelle Molloy (child actress)
- October 14 - Mekai Curtis (actor)
- November 15 - Coco Grayson (child actress)
- November 22 - Auli'i Cravalho (actress and singer)
- January 12 - Marc Davis (artist and animator)
- February 7 - Doug Henning (magician, illusionist, escape artist and politician)
- February 10 - Jim Varney (actor)
- March 13 - Rex Everhart (actor)
- March 16 - Adele Malis-Morey
- April 26 - Joachim Kemmer (voice actor)
- May 10 - Bart the Bear (bear actor)
- May 20 - Jean-Pierre Rampal (flautist)
- June 24 - David Tomlinson (actor)
- July 20 - Eyvind Earle (artist, author and illustrator)
- July 22 - Eric Christmas (stage and screen actor)
- August 5 - Alec Guinness (actor)
- August 25 - Carl Barks (creator of Scrooge McDuck)
- September 26 - Richard Mulligan (actor)
- December 11 - David Lewis (actor)
- December 18 - Nicodemus Stewart (actor)
- December 23 - Billy Barty (actor)
- December 23 - Victor Borge (comedian, conductor and pianist)
- December 26 - Jason Robards (actor)