He appeared as himself in the 1998 Disney television film Safety Patrol, sang the introduction song of the 1996 Hollywood Pictures film Spy Hard, and voiced the singing minstrel in the Lilo & Stitch: The Series episode "Tank". Additionally, he has made multiple parody songs that are based on Lucasfilm's Star Wars, including "The Saga Begins". Yankovic also guest-starred in the Gravity Falls episode "Dungeons, Dungeons, & More Dungeons" as Probabilitor the Annoying, and voiced Dr. Screwball Jones in Wander Over Yonder, Milo Murphy in Milo Murphy's Law, and Preston Change-O in Star vs. the Forces of Evil.
Prior to all of his above-listed roles, his song "Eat It" (a parody of Michael Jackson's "Beat It") was the subject of a music video featured in the DTV TV special Doggone Valentine, and "I Want a New Duck" was used as the opening and closing song for Down and Out with Donald Duck.
In addition, his cover of the George of the Jungle theme was used in the opening credits sequence of the movie adaptation of the TV show, and he was referenced in the Lizzie McGuire episode "Bunkies".